Optimization of Nano-encapsulation on Neonatal Porcine Islet-like Cell Clusters Using Polymersomes

Abstract Researches proving methods for nano-encapsulation of neonatal porcine islet-like cell clusters (NPCCs) using polymersomes (PSomes) formed polymers polyethylene glycol-block-poly lactide. Herein, our studies present efficient procedure with minimal damage and loss NPCCs.We used N-hydroxysuccinimide (NHS) on the N-terminal PSomes to induce binding amine groups in extracellular matrix surrounding NPCCs. F-10 culture medium bovine serum albumin was minimize Finally, we induced cross-linking between bifunctional (NHS-/NH 2 -PSomes). containing 0.25% BSA pH 7.3 minimized NPCCs after as compared basic HBSS buffer (pH 8.0). Also, through conjugation